At each site we measured dark leaf respiration and light saturated leaf photosynthesis using the CIRAS 2 leaf gas exchange analyzer (Company name, web site) on between 15-25 trees once during the wet season and once during the dry season returning to the same tree. We sampled the same trees that were being measured for trunk respiration and randomly cut one sunlit and one shaded branch from each tree which was immediately recut underwater to restore hydraulic connectivity. For dark respiration, we placed the leaf in a black bag for ten minutes prior to measurement. We either controlled CO2 concentrations at 400ppm or used atmospheric air and a buffer to stabilize atmospheric CO2 variability. We controlled the leaf chamber temperature at 25 oC and light levels at 1000 umol m-2 s-1 for light saturated photosynthesis and 0 umol m-2 s-1 for dark respiration.
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I am Professor of Ecosystem Science at Oxford University.
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