Methodology

Ingrowth cores

Fine root productivity is quantified using the ingrowth core (IC) technique (Vogt et al., 1998; Steingrobe et al., 2000; Hendricks et al., 2006), following the methodology described in Metcalfe et al. (2008). These are 30 cm deep cores of root-free soil surrounded by mesh bags in which roots are allowed to grow over a set period of time (e.g. three months), providing estimates of fine root production per unit area and time (Mg ha-1 yr-1). We suggest to install nine ICs per plot, in close proximity to rhizotrons. Soil cores are extracted and soil layers are separated into organic layer (OL) and mineral layer (ML) to avoid mixing the nutrient rich organic soil with the mineral soil.

Fine roots are manually removed every three months using a method which corrects for underestimation of fine root biomass (Metcalfe et al., 2008). Root-free soil is then replaced in each ingrowth core, keeping the structure and composition of the undisturbed soil. Sampled fine roots are rinsed in water to remove soil particles, oven dried at 70°C in the lab until constant mass, weighed and scanned as greyscale images at 600 dpi resolutions. NPPFineRoot is estimated from the quantity of fine root mass produced in the few months interval since the last collection. Fine roots (2 mm diameter. The scanned images may be analyzed using commercially available software (WinRHIZO Pro version 2003 b, Regent Instruments, Canada), to calculate fine root length and surface area, according to the methodology described in Metcalfe et al. (2008).

Organic layer depth and fine root mass measurements from 25 points for each plots should complement this data.

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Photos and images

Medium_p1020962
Uploaded 26 Mar 2014 by Jhon del Aguila Pasquel. Copyright © 2017.

Hello again,

As part of my job at Peruvian Amazon Research Institute (IIAP), the last December it was organized a training workshop in GEM methods for cuantifying carbon stocks and fluxes in tropical forests. Several students and professionals with climate change interest learnt our protocols.

Pictures show part of the data collection and analysis.

Jhon

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